Janet Yee

PhD

Associate Professor, Department of Biology, Trent University

Location

Peterborough, Ontario

Keywords

Giardia, molecular biology, biochemistry, gene expression, transcription

Research interests

The focus in our laboratory is to study the biology of the protozoan parasite, Giardia intestinalis, by using molecular and biochemical approaches. We have expertise in: gene cloning; purification of DNA, RNA and proteins; genetic transformation by the electroporation method; luciferase gene reporter assay; gel mobility shift assay; RT-qPCR; flow cytometry; immunofluorescence microscopy, and centrifugal counterflow elutriation. Our research includes the study of this parasite’s developmental stages, its regulation of gene expression and its cell cycle. We also have ongoing collaborations to analyze heme proteins and their functions in this parasite. The ultimate goal is to identify unique biological aspects of this parasite that can be exploited to develop improved and more effective treatments for giardiasis and to decrease its transmission.

Expertise in

Cryptosporidiosis

Food

Genomics

Giardiasis

Treatment

Relevant publications

R. Pazdzior, Z. Yang, M. Mesbahuddin, J. Yee, A. van der Est, and S. Rafferty. Low reduction potential cytochrome b5 isotypes of Giardia intestinalis, J. Exp. Parasitol 157: 197-201 (2015).

C. Reaume, B. Moore, P. Hernandez, A. Ruzzini, M. Chlebus, M. Wasserman and J. Yee. Evaluation of drugs and stationary growth on the cell cycle of Giardia intestinalis, Mol. Biochem. Parasitol. 187: 72-76 (2013).

A.J. Hudson, A. Moore, D. Elniski, J. Joseph, J. Yee, and A.G. Russell. Evolutionarily divergent spliceosomal snRNAs and a conserved non-coding RNA processing motif in Giardia lamblia, Nucl. Acids Res 40:10995-1008 (2012).

S. Alam, J. Yee, M. Couture, S.J. Takayama, W.H. Tseng, A.G. Mauk, and S. Rafferty. Cytochrome b5 from Giardia lamblia, Metallomics 4: 1255-1261 (2012).

S.W. Roy, A.J. Hudson, J. Joseph, J. Yee, and A.G. Russell. Numerous fragmented spliceosomal introns, AT-AC splicing, and an unusual dynein gene expression pathway in Giardia lamblia, Mol Biol Evol. 29:43-9 (2012).

J. Yee and J. Joseph. Methods for Giardia transfection and gene expression. In Giardia: A Model Organism. (H. Lujan and S. Svard Eds), SpringerWienNewYork, New York, (2011). ISBN 3709101972, 9783709101971.

S. Rafferty, B. Luu, R.E. March, J. Yee. Giardia lamblia encodes a functional flavohemoglobin. Biochem. Biophys. Res. Commun. 399: 347-351 (2010).

J. Yee, A. Tang, W.L. Lau, H. Ritter, D. Delport, M. Page, R. Adam, M. Muller and G. Wu, The core histone genes of Giardia intestinalis: genomic organization, promoter structure, and expression. BMC Mol. Biol. 8: 26-40 (2007).

Contact

Chemical Sciences Building

1600 West Bank Drive

Peterborough, ON K9J 7B8

jyee@trentu.ca